Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Organization01:13

Protein Organization

Overview
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting range expansion of three tropical fruit flies and risks to pome and stone fruit production in temperate regions.

Journal of economic entomology·2026
Same author

Functionalization Enhanced Phase Separation in PS-b-PVP Derived Polyzwitterionic Block Copolymers.

Macromolecular rapid communications·2026
Same author

In vitro activity of cefiderocol and sulbactam-durlobactam against clinical-isolated carbapenem-resistant Acinetobacter baumannii from China.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Early-life exposure to 27.5 GHz 5G millimeter-wave radiation induces skin-related biological responses in mice.

Scientific reports·2026
Same author

Substrate-Controlled Relay Carbonylation Coupled with Regioselective C=C Isomerization.

Organic letters·2026
Same author

Application of escitalopram population pharmacokinetic repository: a step to precision dosing.

Translational psychiatry·2026
Same journal

Predicting Nirmatrelvir Resistance in SARS-CoV-2 M<sup>pro</sup> Mutants with an Integrated Computational Framework.

The journal of physical chemistry. B·2026
Same journal

From Cation Solvation to Anion Coordination: Lewis-Acidic Boranes Enable Halide Salt Electrolytes.

The journal of physical chemistry. B·2026
Same journal

In Vitro-Prepared A30P Alpha-Synuclein Fibrils Adopt the Conserved and Disease-Relevant Greek Key Fold.

The journal of physical chemistry. B·2026
Same journal

Metastructure Analysis of Self-Assembled Nanocubes with Different Equatorial Methyl Groups Based on Molecular Dynamics Simulations.

The journal of physical chemistry. B·2026
Same journal

A Cocoordinated <sup>1</sup>H Internal Reference Quantifies Proton-Exchange Bias in Coordinated-Water Diffusion.

The journal of physical chemistry. B·2026
Same journal

Unveiling Electrolyte-Dependent Coordination Site Dynamics for Redox Mediator Design in Lithium-O<sub>2</sub> Batteries: Exchange vs Rearrangement.

The journal of physical chemistry. B·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Lysozyme protein solution with an intermediate range order structure.

Yun Liu1, Lionel Porcar, Jinhong Chen

  • 1NCNR, National Institute of Standards and Technology, Gaithersburg, Maryland, United States. yunliu@nist.gov

The Journal of Physical Chemistry. B
|December 1, 2010
PubMed
Summary
This summary is machine-generated.

The low-Q peak in lysozyme solutions does not indicate cluster formation but rather intermediate-range order. Dynamic analysis reveals that while clusters form, they are transient with finite lifetimes.

More Related Videos

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
08:56

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Published on: March 25, 2017

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

Related Experiment Videos

Last Updated: Jun 6, 2026

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
08:56

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Published on: March 25, 2017

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Published on: September 23, 2020

Area of Science:

  • Colloidal science
  • Biophysics
  • Soft matter physics

Background:

  • Equilibrium cluster formation is a key phenomenon in colloidal and protein solutions.
  • A low-Q correlation peak in scattering patterns was previously attributed to cluster-cluster correlations in lysozyme solutions.

Purpose of the Study:

  • To investigate the origin of the low-Q peak in lysozyme solutions.
  • To determine if the observed peak signifies cluster formation or another structural feature.
  • To characterize the dynamics and lifetime of clusters in concentrated lysozyme solutions.

Main Methods:

  • Quantitative analysis of small-angle neutron scattering (SANS) and neutron spin echo (NSE) data.
  • Application of statistical mechanics models to scattering data.
  • Measurement of diffusion coefficients using NSE and nuclear magnetic resonance (NMR).

Main Results:

  • The low-Q peak is not a signature of cluster formation but arises from intermediate-range order.
  • This order is driven by short-range attraction and long-range repulsion.
  • Clusters observed in concentrated solutions are not permanent and possess a finite lifetime.

Conclusions:

  • The interpretation of low-Q peaks in scattering data requires careful consideration of underlying structures.
  • Lysozyme solutions exhibit complex structural organization beyond simple clustering.
  • Dynamic studies reveal transient cluster behavior in concentrated protein solutions.