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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Related Experiment Video

Updated: Jun 6, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Published on: May 16, 2017

Single-particle applications at intermediate resolution.

Bettina Böttcher1, Katharina Hipp

  • 1University of Edinburgh, United Kingdom.

Advances in Protein Chemistry and Structural Biology
|December 1, 2010
PubMed
Summary

Electron microscopy and single-particle image processing reveal the architecture of biological assemblies. This method determines structures at intermediate resolution, enabling pseudo-atomic modeling and understanding of complex dynamics.

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Last Updated: Jun 6, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
13:28

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Published on: May 16, 2017

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Area of Science:

  • Structural Biology
  • Biophysics
  • Molecular Imaging

Background:

  • Biological assemblies are complex structures crucial for cellular function.
  • Determining the structure of these large complexes is essential for understanding their mechanisms.

Purpose of the Study:

  • To review the methodology of electron microscopy and single-particle image processing for biological assembly structure determination.
  • To illustrate how intermediate-resolution structures inform pseudo-atomic modeling and functional dynamics.

Main Methods:

  • Utilizing electron microscopy for image acquisition of biological assemblies.
  • Applying single-particle image processing and coherent averaging techniques.
  • Achieving 3D structure determination at 1-3 nm resolution.

Main Results:

  • Enables mapping of individual subunits within biological assemblies.
  • Provides insights into the architecture and quaternary structure of complexes.
  • Facilitates the creation of pseudo-atomic models using known subunit structures.

Conclusions:

  • Electron microscopy with image processing is a powerful tool for elucidating the structure of multicopy biological complexes.
  • Intermediate-resolution structures are foundational for understanding assembly architecture, dynamics, and function.