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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Published on: August 5, 2011

E2f1-3 are critical for myeloid development.

Prashant Trikha1, Nidhi Sharma, Rene Opavsky

  • 1College of Medicine and Public Health, Ohio State University, Columbus, Ohio 43210, USA.

The Journal of Biological Chemistry
|December 1, 2010
PubMed
Summary
This summary is machine-generated.

E2f1-3 proteins are essential for myeloid development, acting first as repressors for progenitor survival and later as activators for macrophage proliferation. These dual roles are critical for hematopoietic development in vivo.

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Related Experiment Videos

Last Updated: Jun 6, 2026

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Biology

Background:

  • Hematopoietic development relies on precise regulation of cell differentiation and the cell cycle.
  • Current models identify E2f activators (E2f1, E2f2, E2f3) as key drivers of S phase entry and progression.

Purpose of the Study:

  • To investigate the role of E2f1-3 in myeloid progenitor and macrophage development.
  • To elucidate the distinct functions of E2f1-3 during different stages of myeloid differentiation in vivo.

Main Methods:

  • Conditional gene knock-out strategies in mice.
  • Analysis of myeloid progenitor and macrophage populations.
  • Assessment of cell survival and proliferation markers.

Main Results:

  • E2f1-3 are dispensable for early myeloid progenitor proliferation.
  • E2f1-3 function as transcriptional repressors essential for CD11b(+) myeloid progenitor survival.
  • E2f1-3 act as transcriptional activators promoting CD11b(+) macrophage proliferation in response to CSF1-Myc signals.

Conclusions:

  • E2f1-3 exhibit dual functions in myeloid development: repressing survival in early progenitors and activating proliferation in macrophages.
  • These findings reveal a critical role for E2f1-3 in a specific signaling cascade essential for in vivo myeloid development.