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Updated: Jun 6, 2026

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Published on: February 7, 2018

High-resolution simultaneous voltage and Ca2+ imaging.

Kaspar E Vogt¹, Stephan Gerharz, Jeremy Graham

¹Division of Pharmacology and Neurobiology, Biozentrum-University of Basel, Basel, Switzerland.

The Journal of Physiology

|December 1, 2010

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This study introduces a new apparatus for simultaneous voltage and calcium (Ca2+) imaging in neurons. The technique offers high resolution and minimal optical cross-talk, enabling detailed study of cellular electrical and chemical activity.

Area of Science:

Neuroscience
Biophysics
Cellular Biology

Background:

Understanding the interplay between electrical and chemical signaling is crucial for neuroscience.
Current techniques often face limitations in resolution, cross-talk, or photodamage when measuring voltage and calcium simultaneously.
Sub-cellular level correlation of these activities requires advanced imaging methodologies.

Purpose of the Study:

To develop and validate a novel apparatus for simultaneous voltage and Ca(2+) imaging.
To achieve single-trial resolution at the sub-micron level.
To minimize optical cross-talk and photodamage during measurements.

Main Methods:

Development of a novel apparatus for simultaneous voltage and Ca(2+) measurements.
Utilizing advanced optical and electrophysiological recording techniques.

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Last Updated: Jun 6, 2026

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Published on: February 7, 2018

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca²⁺ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

Testing the apparatus on individual neurons in brain slices and cultured neuronal networks.

Main Results:

The apparatus successfully obtained simultaneous voltage and Ca(2+) measurements with single-trial resolution.
Measurements were achieved from sites as small as a few microns.
Negligible optical cross-talk and photodamage were observed between the voltage and Ca(2+) signals.

Conclusions:

The developed apparatus provides a powerful tool for correlating electrical and chemical activity at the sub-cellular level.
This technique overcomes previous limitations, paving the way for novel physiological investigations.
Enables detailed study of neuronal function and network dynamics.