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Updated: Aug 5, 2026
A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection
Published on: February 20, 2020
[The modelling of a Pseudomonas aeruginosa wound process]
Abstract:
P. aeruginosa wound infection was induced in white mice to test new preparations against P. aeruginosa. This model ensures the nearest approximation to the course of P. aeruginosa chronic infection, i.e. it reproduces the focus of inflammation and the prolonged course of the disease (the positive decision on application No. 4, 324, 555 of November 2, 1987, has been obtained). The essence of the method consists in obtaining the model of P. aeruginosa wound infection by a combined trauma of the skin (burn and incision): P. aeruginosa is introduced in a dose of 6 X 10(9)-8 X 10(9) microbial cells into the burn blister through the incision made 3 hours after the burn and then 20-24 hours later in a dose of 10(9)-2 X 10(9) microbial cells, introduced under the crust formed by that time.
Insights
A novel mouse model for Pseudomonas aeruginosa wound infection was developed to mimic chronic infections. This model, involving combined burn and incision trauma, allows for testing new anti-bacterial preparations.
Area of Science:
- Microbiology
- Infectious Diseases
- Animal Models
Background:
- Pseudomonas aeruginosa (P. aeruginosa) is a significant pathogen causing chronic wound infections.
- Developing accurate animal models is crucial for evaluating novel therapeutic strategies against persistent bacterial infections.
Purpose of the Study:
- To establish a reliable mouse model that closely approximates the chronic P. aeruginosa wound infection.
- To create a reproducible platform for testing the efficacy of new anti-bacterial preparations.
Main Methods:
- Induction of P. aeruginosa wound infection in white mice using a combined burn and incision trauma model.
- Introduction of P. aeruginosa in specific microbial cell doses into burn blisters and under crusts at defined time points post-trauma.
Main Results:
- The developed model successfully reproduces the inflammatory focus characteristic of chronic P. aeruginosa infections.
- The model facilitates a prolonged disease course, closely mimicking clinical scenarios.
Conclusions:
- The combined burn and incision trauma model provides a robust method for inducing P. aeruginosa wound infection in mice.
- This model serves as a valuable preclinical tool for assessing new treatments against P. aeruginosa chronic infections.
