Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Retroviruses02:33

Retroviruses

Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Viral Hepatitis I: Introduction01:28

Viral Hepatitis I: Introduction

Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion of food...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immune response to rotavirus vaccines in low- and middle-income countries: Challenges and perspectives.

Human vaccines & immunotherapeutics·2026
Same author

Different Fc scaffolds enhance the breadth of <i>in vitro</i> neutralization of the same Fab against different Rotavirus strains.

Frontiers in immunology·2026
Same author

The Next Upgrade for Surgical Procedure-Based Assessments: Clinical Video Footage.

Cureus·2026
Same author

Contribution of the EuroRotaNet surveillance network to rotavirus strain surveillance in Europe.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2025
Same author

Enhancing infectious intestinal disease diagnosis through metagenomic and metatranscriptomic sequencing of 1000 human diarrhoeal samples.

Genome medicine·2025
Same author

Age-specific immunity to rotavirus infection and the risk of disease before and after rotavirus vaccine introduction in the United Kingdom: an observational, seroepidemiological study.

medRxiv : the preprint server for health sciences·2025

Related Experiment Video

Updated: Jun 6, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Rotaviruses.

Jim Gray1, Miren Iturriza-Gómara

  • 1Enteric Virus Unit, Virus Reference Department, Centre for Infections, Health Protection Agency, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2010
PubMed
Summary
This summary is machine-generated.

Detecting and characterizing rotaviruses, including VP6 proteins and diverse strains, is crucial for developing effective vaccines. These methods identify novel and zoonotic strains, aiding public health efforts.

More Related Videos

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

Related Experiment Videos

Last Updated: Jun 6, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Rotaviruses are common causes of gastroenteritis.
  • Detection methods include morphology visualization, immunological assays, and molecular techniques.
  • Understanding rotavirus diversity is essential for vaccine development.

Purpose of the Study:

  • To outline methods for rotavirus detection and characterization.
  • To emphasize the importance of identifying diverse rotavirus strains for vaccine development.
  • To describe techniques for identifying novel, reassortant, and zoonotic rotavirus strains.

Main Methods:

  • Visualizing rotavirus morphology.
  • Detecting rotavirus proteins (e.g., VP6) using immunological methods.
  • Analyzing rotavirus genome diversity via polyacrylamide gel electrophoresis, reverse transcription, and PCR.
  • Characterizing genes encoding structural proteins (VP4, VP7, VP6) and non-structural protein (NSP4).

Main Results:

  • Abundant virus particles in clinical samples favor VP6 protein detection.
  • Molecular methods are valuable for characterizing diverse circulating rotavirus populations.
  • Combined detection and characterization methods identify novel strains from genetic reassortment and point mutations.
  • Genomic and antigenic diversity of human and animal rotaviruses can be identified.

Conclusions:

  • Effective rotavirus vaccine development requires comprehensive detection and characterization of circulating strains.
  • Identification of diverse rotaviruses, including zoonotic strains, is critical for public health.
  • The described methods enable the identification of novel rotavirus strains arising from genetic reassortment and mutation.