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Updated: Jun 6, 2026
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Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
Published on: January 9, 2017
Measuring the hydrodynamic size of nanoparticles in aqueous media using batch-mode dynamic light scattering
Vincent A Hackley1, Jeffrey D Clogston
1Ceramics Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2010
Abstract:
Particle size characterization is of particular importance to nanomedicine. The size similarity of nanoparticles to biological moieties is believed to impart many of their unique medical properties. Here we present a method for sample preparation and the determination of mean nanoparticle size (hydrodynamic diameter) using batch-mode dynamic light scattering (DLS) in dilute aqueous suspensions. We then demonstrate this method for 30 nm colloidal gold.
