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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
Published on: December 18, 2014
Flow cytometry: an introduction.
1Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center and Department of Physiology, Dartmouth Medical School, Lebanon, NH, USA. Alice.L.Givan@Dartmouth.edu
Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2010
Summary
Flow cytometry instruments analyze individual cells by illuminating them and detecting emitted signals. This technology enables cell sorting and detailed data analysis for biological research.
Area of Science:
- Biotechnology
- Cell Biology
- Analytical Chemistry
Background:
- Flow cytometry is a powerful technique for analyzing individual cells or particles.
- It relies on illuminating cells in flow and detecting resulting signals.
Purpose of the Study:
- To provide a comprehensive overview of flow cytometry principles and applications.
- To detail the components and processes involved in flow cytometer operation.
Main Methods:
- Illumination of single cells in fluidic streams.
- Detection and correlation of light signals emitted by cells.
- Digital conversion and computer-based data analysis.
- Cell sorting based on detected characteristics.
Main Results:
- Detailed explanation of cell characteristics suitable for flow cytometry.
- Description of illumination, fluidics, and signal detection mechanisms.
- Overview of data processing, analysis, and cell sorting capabilities.
Conclusions:
- Flow cytometry is a versatile tool for cell analysis and sorting.
- Understanding its principles is key to its effective application in research.
- This chapter serves as an introduction to the broader field of cytometry.
