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Related Experiment Video

Updated: May 1, 2026

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Published on: February 8, 2016

Flow-based combinatorial antibody profiling: an integrated approach to cell characterization.

Shane Bruckner¹, Ling Wang, Ruiling Yuan

¹BD Biosciences, San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 1, 2010

Summary

BD FACS™ CAP (combinatorial antibody profile) offers rapid human cell surface protein analysis. This high-throughput flow cytometry tool efficiently characterizes 229 markers per cell type using automated workflows.

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Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Characterizing human cell surface protein expression is crucial for understanding cellular function and disease.
High-throughput screening methods are needed to efficiently analyze large numbers of markers and cell types.

Purpose of the Study:

To introduce and describe the BD FACS™ CAP (combinatorial antibody profile) system as a novel tool for rapid characterization of human cell surface protein expression.
To highlight the capabilities of the system in analyzing a comprehensive panel of 229 surface markers.

Main Methods:

Utilizes semi-automated high-throughput flow cytometry with a 96-well plate configuration.
Employs 229 directly conjugated antibodies in three-color cocktails for marker analysis.
Incorporates automated liquid handling for staining, automated flow cytometry for data acquisition, and standardized algorithms for data analysis.

Main Results:

The BD FACS™ CAP system enables efficient characterization of individual cell surface markers.
The semi-automated workflow allows for rapid analysis of multiple cell types.
Standardized algorithms ensure accurate calculation of marker expression levels for each cell type.

Conclusions:

BD FACS™ CAP provides a highly efficient and semi-automated solution for comprehensive human cell surface protein profiling.
The system's integrated automated processes streamline the workflow from staining to data analysis.
This tool facilitates rapid characterization of cell surface markers, supporting various research applications.