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Updated: Jun 6, 2026
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Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
Published on: February 1, 2017
Flow cytometry assays in primary immunodeficiency diseases.
Maurice R G O'Gorman1, Joshua Zollett, Nicolas Bensen
1Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. mogorman@childrensmemorial.org
Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2010
Summary
Primary immunodeficiency diseases (PIDs) involve genetic mutations affecting the immune system. Flow cytometry detects these abnormalities in blood cells, focusing on lymphocyte subsets, markers, and leukocyte functions.
Area of Science:
- Immunology
- Genetics
- Hematology
Background:
- Primary immunodeficiency diseases (PIDs) represent a diverse group of genetic disorders impacting the immune system.
- Mutations in PID genes often manifest as detectable abnormalities in peripheral blood cells.
- Flow cytometry is a key technology for identifying these cellular abnormalities.
Purpose of the Study:
- To review essential flow cytometry procedures for diagnosing primary immunodeficiency diseases.
- To provide a global overview of immune abnormalities detectable by flow cytometry.
- To focus on three core areas: lymphocyte subset, marker, and leukocyte function abnormalities.
Main Methods:
- Review of established flow cytometry protocols for PID diagnosis.
- Focus on assays detecting lymphocyte subset enumeration and phenotyping.
- Examination of methods for assessing leukocyte function.
Main Results:
- Flow cytometry enables detection of lymphocyte subset abnormalities.
- Analysis of lymphocyte "marker" abnormalities is crucial for PID diagnosis.
- Assessment of leukocyte function provides insights into immune system defects.
Conclusions:
- Specific flow cytometry procedures offer a comprehensive approach to PID diagnosis.
- These methods are vital for identifying a wide range of immune system defects.
- The reviewed techniques aid in understanding the complexity of primary immunodeficiency diseases.
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01:23Flow Cytometry
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
In...
01:25Immunodeficiency Diseases
Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
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