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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 6, 2026

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Flow cytometric analysis of microparticles.

Henri C van der Heyde1, Irene Gramaglia, Valéry Combes

  • 1Cell Analysis Core Facility, Flow Cytometry, La Jolla Infectious Disease Institute, San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2010
PubMed
Summary

Cell-derived microparticles (MPs) are key for cell signaling and disease. This study presents flow cytometry methods to quantify MP numbers and surface proteins, improving disease diagnostics.

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Last Updated: Jun 6, 2026

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Area of Science:

  • Cell Biology
  • Immunology
  • Biotechnology

Background:

  • Cell-derived microparticles (MPs) are vital for intercellular communication in health and disease.
  • MPs, distinct from exosomes and apoptotic bodies, carry surface proteins from their parent cells.
  • Current methods like electron microscopy and ELISA lack precision in quantifying MPs and their surface markers.

Purpose of the Study:

  • To develop and validate a flow cytometry-based method for assessing MP number and phenotype.
  • To address limitations of conventional flow cytometry in MP analysis.
  • To introduce an advanced imaging flow cytometry approach for comprehensive MP characterization.

Main Methods:

  • Utilized conventional flow cytometry to quantify microparticle (MP) numbers and analyze their surface protein expression.
  • Identified and described limitations associated with standard flow cytometry for MP analysis.
  • Developed and presented an imaging flow cytometry technique to overcome these limitations.

Main Results:

  • Established a flow cytometry protocol for enumerating MPs and determining their phenotype.
  • Highlighted critical caveats of conventional flow cytometry in MP quantification and characterization.
  • Demonstrated the efficacy of imaging flow cytometry in providing a more detailed analysis of MPs.

Conclusions:

  • Flow cytometry offers a viable approach for analyzing cell-derived microparticles (MPs).
  • Imaging flow cytometry provides enhanced capabilities for precise MP quantification and phenotyping.
  • These advancements facilitate a deeper understanding of MP roles in physiological and pathological processes.