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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Photoluminescence: Applications01:14

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
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Updated: Jun 6, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

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Published on: April 7, 2023

O(2)/pH multisensor based on one phosphorescent dye.

Nicolas B Borchert, Gelii V Ponomarev, Joe P Kerry

    Analytical Chemistry
    |December 2, 2010
    PubMed
    Summary

    A novel sensor uses a special dye to measure both oxygen and pH simultaneously. This innovative approach offers accurate, continuous monitoring with potential for sensing other substances.

    Area of Science:

    • Analytical Chemistry
    • Materials Science

    Background:

    • Development of a novel sensor system utilizing a phosphorescent metalloporphyrin dye embedded within a polymeric membrane.
    • The sensor is designed for the simultaneous measurement of two critical analytes: oxygen and pH.

    Discussion:

    • The bifunctional dye exhibits dual sensing mechanisms: oxygen (O(2)) quenches phosphorescence intensity and lifetime, while protonation alters absorption spectra and reduces phosphorescence.
    • Two distinct measurement strategies are proposed: (i) simultaneous phosphorescence intensity (O(2), pH) and lifetime (O(2)) analysis, and (ii) parallel phosphorescence lifetime (O(2)) and ratiometric absorbance (pH) measurements.
    • The ratiometric absorbance method offers internal referencing and calibration-free operation for both analytes, enhancing sensor reliability.

    Key Insights:

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  • The sensor enables precise quantification and continuous monitoring of oxygen and pH.
  • The phosphorescent dye's response to oxygen and protons allows for dual-analyte detection.
  • Calibration-free sensing is achievable using the ratiometric absorbance and phosphorescence lifetime measurement approach.
  • Outlook:

    • The described sensor technology demonstrates potential for extension to the detection of other analytes.
    • This platform could lead to advancements in multi-analyte sensing systems for environmental and biomedical applications.