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Transduction of a plasmid containing the bacteriophage D3 cos site in Pseudomonas aeruginosa
R Sharp1, E Gertman, M A Farinha
1Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Journal of Bacteriology
|June 1, 1990
Abstract:
Plasmids harboring the cos sequences of bacteriophage D3 can be transferred, by bacteriophage D3, into Pseudomonas aeruginosa by a mechanism which is insensitive to DNase. Transducing activity was separated from the plaque-forming particles by CsCl equilibrium gradient centrifugation. Restriction endonuclease digestion patterns suggest that the transducing particles contain plasmid concatemers.