Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mycobacteria and macrophage activation.

R van Furth1

  • 1Dept. of Infectious Diseases, University Hospital, Leiden, The Netherlands.

Research in Microbiology
|February 1, 1990
PubMed
Summary

Mycobacterium bovis infection activates macrophages but with limited bacterial killing. Interferon-gamma activates macrophages and granulocytes, enhancing the killing of certain bacteria like Mycobacterium fortuitum.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transforming growth factor-beta suppresses interferon-gamma-induced toxoplasmastatic activity in murine macrophages by inhibition of tumour necrosis factor-alpha production.

Parasite immunology·2001
Same author

Protection and humoral immune responses against Bordetella pertussis infection in mice immunized with acellular or cellular pertussis immunogens.

Vaccine·2001
Same author

Enhanced antibody response to pneumococcal polysaccharide vaccine after prior immunization with conjugate pneumococcal vaccine in HIV-infected adults.

Vaccine·2000
Same author

Interleukin-10 has different effects on proliferation of Listeria monocytogenes in livers and spleens of mice.

Infection and immunity·2000
Same author

Antibody response after influenza vaccination in HIV-infected individuals: a consecutive 3-year study.

Vaccine·2000
Same author

Binding of murine antibodies against whole-cell pertussis vaccine or filamentous haemagglutinin by Bordetella pertussis from patients with whooping cough.

Scandinavian journal of immunology·2000

Area of Science:

  • Immunology
  • Microbiology
  • Infectious Diseases

Background:

  • Older concepts regarding macrophage activation and bacterial infection are being re-evaluated.
  • Understanding macrophage and granulocyte responses to mycobacterial infections is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the functional consequences of macrophage activation during Mycobacterium bovis BCG infection.
  • To assess the impact of interferon-gamma and mycobacterial antigens on immune cell bactericidal activity.

Main Methods:

  • Infection of mice with Mycobacterium bovis BCG.
  • In vitro and in vivo activation of macrophages using interferon-gamma.
  • Assessment of macrophage and granulocyte bactericidal/bacteriostatic activity.
  • Analysis of immune response to a 65-kDa mycobacterial protein.

Main Results:

  • Macrophage activation during M. bovis BCG infection showed limited enhancement of bactericidal activity against most bacteria.
  • Interferon-gamma activated macrophages in vitro and in vivo, but without increased bactericidal capacity.
  • A 65-kDa mycobacterial protein induced limited in vivo macrophage activation.
  • Interferon-gamma-stimulated granulocytes demonstrated enhanced killing of ingested M. fortuitum.

Conclusions:

  • Macrophage activation by M. bovis BCG is insufficient to confer broad bactericidal activity.
  • Interferon-gamma's role in enhancing macrophage bactericidal function in vivo requires further investigation.
  • Granulocyte activation by interferon-gamma represents a potential therapeutic avenue for specific bacterial infections.

Related Experiment Videos