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Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Published on: April 18, 2019
1Department of Oral and Maxillofacial Surgery, School of Medicine, Nagoya University, Nagoya Aichi, Japan. mueda@med.nagoya-u.ac.jp
Abstract:
The interaction between stem cells from human exfoliated deciduous teeth (SHEDs)-derived growth factors and human dermal fibroblast (HDF) has been investigated for the first time, as the application of SHEDs for dermal wound healing remains speculative. Stem cells from human exfoliated deciduous teeth have effects on HDFs by increasing collagen synthesis and by activating proliferation and migration activity of HDFs, suggesting that SHEDs or SHED-derived conditioned medium (SH-CM) can be used for the treatment of photoaging. Our results suggest that SHEDs and SH-CM should be constitutionally suited for photoaging treatment. Mainly with secreted growth factors or extracellular matrix proteins, SHEDs contribute to enhanced wound-healing potential of HDFs. Further mechanism studies using neutralizing antibodies against each growth factor may clarify the role of soluble factors of SHEDs in wound-healing process.
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