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Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview

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Related Experiment Video

Updated: Jun 6, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Telomere replication: poised but puzzling.

Shilpa Sampathi1, Weihang Chai

  • 1WWAMI Medical Education Program, Washington State University, Spokane, WA 99210-1495, USA.

Journal of Cellular and Molecular Medicine
|December 3, 2010
PubMed
Summary
This summary is machine-generated.

Accurate chromosome replication is vital for genome stability. This review explores how DNA replication and telomere-specific proteins work together to ensure proper telomere replication, maintenance, and protection.

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Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
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Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

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Last Updated: Jun 6, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
11:21

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Published on: August 30, 2024

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Faithful chromosome replication is crucial for maintaining genome stability.
  • Telomeres, the ends of chromosomes, present unique challenges to DNA replication due to their complex structures and repetitive sequences.
  • Incomplete or aberrant telomere replication can lead to genomic instability and DNA loss.

Purpose of the Study:

  • To review the synergistic actions of DNA replication proteins and telomere protective components in telomere synthesis.
  • To discuss the roles of various replication and telomere-specific binding proteins in ensuring accurate telomere replication.
  • To highlight the importance of these proteins in telomere maintenance and protection.

Main Methods:

  • This is a review article, synthesizing existing research and evidence.
  • It focuses on the functional roles and interactions of proteins involved in DNA replication and telomere biology.
  • Literature search and analysis of published studies on telomere replication and maintenance.

Main Results:

  • Compelling evidence suggests synergistic interactions between DNA replication machinery and telomere protective factors.
  • Specific replication and telomere-binding proteins play critical roles in the accurate duplication of telomeric DNA.
  • These proteins are essential for preventing telomere dysfunction and maintaining overall genome integrity.

Conclusions:

  • The coordinated action of replication and telomere-specific proteins is fundamental for accurate telomere replication.
  • Understanding these protein interactions provides insights into telomere maintenance and protection mechanisms.
  • Dysregulation of these processes can have significant implications for genome stability and cellular health.