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Interaction of Sesbania mosaic virus movement protein with the coat protein--implications for viral spread

Soumya Roy Chowdhury1, Handanahal Subbarao Savithri

  • 1Department of Biochemistry, Indian Institute of Science, Bangalore, India.

The FEBS Journal
|December 3, 2010
PubMed

Insights

Sesbania mosaic virus movement protein (MP) interacts with the virus's native virus (NV) via its N-terminal segment. This interaction is crucial for sobemovirus cell-to-cell movement, potentially involving the coat protein.

Area of Science:

  • Plant Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Sesbania mosaic virus (SeMV) is a positive-sense, single-stranded RNA virus in the Sobemovirus genus.
  • Its movement protein (MP) shares limited similarity with other genera but shows homology within its genus.

Purpose of the Study:

  • To elucidate the mechanism of cell-to-cell movement in sobemoviruses.
  • To investigate the interaction between SeMV MP and the native virus (NV).

Main Methods:

  • Cloning, overexpression, and purification of SeMV MP in E. coli.
  • ELISA and pull-down assays to study MP-NV interaction.
  • Yeast two-hybrid assays and analysis of MP deletion mutants.

Main Results:

  • SeMV MP directly interacts with NV in a concentration- and pH-dependent manner.
  • The N-terminal 49 amino acid segment of SeMV MP is essential for NV interaction.
  • In vitro and yeast two-hybrid results confirm MP-NV interaction.

Conclusions:

  • SeMV cell-to-cell movement likely requires the MP and NV/coat protein.
  • This finding contributes to understanding sobemovirus replication and spread mechanisms.

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