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Related Experiment Videos

Oscillatory CO2 evolution in glycolysing yeast extracts.

J Das1, H Timm, H G Busse

  • 1Institute of Biochemistry, University of Kiel, F.R.G.

Yeast (Chichester, England)
|May 1, 1990
PubMed
Summary

Yeast extracts show oscillating carbon dioxide production during glycolysis. High CO2 output correlates with high NADH levels, indicating increased glycolytic flux.

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Area of Science:

  • Biochemistry
  • Metabolic Engineering
  • Systems Biology

Background:

  • Glycolysis is a fundamental metabolic pathway for energy production in cells.
  • Understanding the dynamics of glycolysis, particularly during oscillations, is crucial for comprehending cellular regulation.
  • Yeast cytoplasmic extracts provide a model system to study glycolytic oscillations under controlled conditions.

Purpose of the Study:

  • To investigate the rate of carbon dioxide (CO2) production during glycolytic oscillations in yeast extracts.
  • To correlate CO2 production rates with NADH (nicotinamide adenine dinucleotide) levels.
  • To elucidate the relationship between NADH concentration and glycolytic flux.

Main Methods:

  • Utilized membrane inlet mass spectrometry to measure CO2 production rates in real-time.
  • Employed an open system with continuous glucose infusion to sustain glycolytic oscillations.
  • Monitored NADH light absorption to track oscillations of the relaxation type.

Main Results:

  • CO2 production exhibited cyclical oscillations, peaking at approximately 100 mumol/ml/h and falling to 50 mumol/ml/h.
  • NADH levels also oscillated, showing a relaxation-type pattern.
  • High CO2 production phases coincided with high NADH levels, suggesting increased glycolytic flux during these periods.

Conclusions:

  • Glycolytic oscillations in yeast extracts are characterized by fluctuating CO2 production and NADH levels.
  • The observed correlation provides evidence that high NADH concentrations drive increased flux through glycolysis.
  • Despite sustained oscillations, only 50% of infused glucose was metabolized to CO2, indicating complex metabolic regulation.

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