Related Experiment Video
Updated: Jun 6, 2026

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
Published on: April 12, 2019
The phosphorus fractions and alkaline phosphatase activities in sludge
Chunsheng Xie1, Jie Zhao, Jie Tang
1Department of Environmental Engineering, Zhejiang University, Hangzhou, PR China.
Abstract:
The alkaline phosphatase activities (APA) and phosphorus fractions in activated sludge during wastewater treatment were studied. Our results showed that the phosphorus concentration and fractions in activated sludge were highly correlated with the characteristics of influents. Inorganic phosphorus (IP) and non-apatite inorganic phosphorus (NAIP) were the main phosphorus fractions of sludge. A larger phosphorus concentration was found in activated sludge due to the more readily mobilizable and bio-available forms. The APA in sludge was directly correlated with mixed liquor suspended solids (MLSS) in activated sludge. The APA in the sludge is implicated the depletion of organic phosphorus forms in sludge, whilst also implying its less inhibition of inorganic phosphorus in sludge. The APA and phosphorus fractions in different sludge samples from the same wastewater treatment plant were quite stable. This stability shows their tight interactions in sludge.
Related Concept Videos
Factors Affecting Solubility
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
The Phosphorus Cycle

