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Updated: Jun 6, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Microbial behaviors involved in cake fouling in membrane bioreactors under different solids retention times
Bing Wu1, Shan Yi, Anthony G Fane
1Singapore Membrane Technology Centre, Nanyang Technological University, Singapore, Singapore. wubing@ntu.edu.sg
Abstract:
Biomass characteristics and membrane performances in the MBRs operated at a high flux of 30 L/m(2) h under different SRTs (10, 30 days, and infinity) were monitored. Results showed that more serious cake-fouling happened in the SRT-infinity MBR, which correlated with the activated sludge characteristics such as smaller floc size and greater EPS amount. DGGE analysis indicated that the microbial community shifted in different ways under various SRTs, which also influenced EPS productions in the MBRs. Different microbial communities were developed on the membrane surfaces at various operating stages and SRTs. Possibly, the activated sludge characteristics (such as MLSS concentration, EPS properties) and hydrodynamic conditions influenced by the SRTs were associated with cake layer development and membrane fouling propensity. Insight into the EPS characteristics and deposition behaviors of bacterial flocs will be crucial to explore appropriate biofouling control strategies in MBRs.
Insights
Longer sludge retention times (SRTs) in membrane bioreactors (MBRs) increase cake-fouling due to smaller flocs and more extracellular polymeric substances (EPS). Microbial community shifts also impact EPS production and membrane performance.
Area of Science:
- Environmental Engineering
- Biotechnology
- Water Treatment
Background:
- Membrane bioreactors (MBRs) are effective wastewater treatment systems.
- Membrane fouling remains a significant challenge in MBR operations.
- Sludge retention time (SRT) is a critical operational parameter influencing MBR performance.
Purpose of the Study:
- To investigate the impact of different sludge retention times (SRTs) on biomass characteristics and membrane performance in MBRs.
- To understand the relationship between activated sludge properties, microbial community structure, and membrane fouling.
- To identify factors contributing to cake-fouling and biofouling propensity.
Main Methods:
- Operation of MBRs at a high flux (30 L/m² h) with varying SRTs (10, 30 days, and infinity).
- Monitoring of biomass characteristics (floc size, EPS content, MLSS concentration).
- Analysis of microbial community shifts using DGGE (Denaturing Gradient Gel Electrophoresis).
- Assessment of membrane performance and cake-fouling development.
Main Results:
- The MBR operated at an infinite SRT exhibited more severe cake-fouling.
- This fouling correlated with smaller activated sludge floc size and increased extracellular polymeric substances (EPS) amount.
- DGGE analysis revealed distinct microbial community shifts across different SRTs, influencing EPS production.
- Diverse microbial communities developed on membrane surfaces under various SRTs and operating stages.
Conclusions:
- Activated sludge characteristics (MLSS, EPS properties) and hydrodynamics, influenced by SRTs, are linked to cake layer development and membrane fouling.
- Understanding EPS characteristics and bacterial floc deposition is crucial for developing effective biofouling control strategies in MBRs.
- Optimizing SRT is essential for mitigating membrane fouling and ensuring efficient MBR operation.
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