Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 6, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Published on: June 14, 2012

Creating zinc finger nucleases using a modular-assembly approach.

Matthew Porteus

    Cold Spring Harbor Protocols
    |December 3, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Epigenetic landscape, key transcriptional regulators, and in vivo identification of human Tr1 cells.

    Science advances·2026
    Same author

    Engineered hematopoietic stem cells give rise to therapeutic antibody secreting B cells.

    Research square·2026
    Same author

    Harnessing DNA barcoding to enhance and sustain polyclonality in gene-edited hematopoietic stem cells.

    Molecular therapy. Methods & clinical development·2025
    Same author

    Overcoming barriers to commercially pre-viable gene and cell therapies for rare and ultra-rare diseases.

    Molecular therapy : the journal of the American Society of Gene Therapy·2025
    Same author

    Global Observatory for Genome Editing Summit Statement: Call for a Charter on Emerging Technologies and Human Dignity.

    The CRISPR journal·2025
    Same author

    Immune composition of the mononuclear cell fraction of human umbilical cord blood.

    Frontiers in immunology·2025

    This study introduces a method to engineer zinc finger nucleases (ZFNs) for precise genome editing in mammalian somatic cells. This technique enables targeted DNA double-strand breaks, significantly enhancing homologous recombination for genetic manipulation.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biotechnology

    Background:

    • Homologous recombination is a precise genome manipulation tool, widely used in model organisms but limited in mammalian somatic cells.
    • Targeted DNA double-strand breaks are key to stimulating homologous recombination in mammalian genomes.
    • Zinc finger nucleases (ZFNs) are engineered proteins capable of creating such targeted breaks.

    Purpose of the Study:

    • To describe a protocol for assembling novel three-finger proteins.
    • To detail the creation of new zinc finger nucleases (ZFNs).
    • To enable precise genome editing in mammalian somatic cells via ZFN-mediated homologous recombination.

    Main Methods:

    • Utilizing a polymerase chain reaction (PCR) technique for protein assembly.

    More Related Videos

    Mouse Genome Engineering Using Designer Nucleases
    12:04

    Mouse Genome Engineering Using Designer Nucleases

    Published on: April 2, 2014

    Automated Robotic Liquid Handling Assembly of Modular DNA Devices
    11:22

    Automated Robotic Liquid Handling Assembly of Modular DNA Devices

    Published on: December 1, 2017

    Related Experiment Videos

    Last Updated: Jun 6, 2026

    Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
    09:11

    Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

    Published on: June 14, 2012

    Mouse Genome Engineering Using Designer Nucleases
    12:04

    Mouse Genome Engineering Using Designer Nucleases

    Published on: April 2, 2014

    Automated Robotic Liquid Handling Assembly of Modular DNA Devices
    11:22

    Automated Robotic Liquid Handling Assembly of Modular DNA Devices

    Published on: December 1, 2017

  • Designing and constructing artificial zinc finger proteins fused to nuclease domains.
  • Generating gene-specific DNA double-strand breaks in mammalian genomes using ZFNs.

    • Main Results:

    • Successful assembly of new three-finger proteins.
    • Creation of functional zinc finger nucleases (ZFNs).
    • Demonstrated potential for ZFNs to stimulate homologous recombination in mammalian somatic cells.

    Conclusions:

    • The described PCR-based protocol facilitates the creation of novel ZFNs.
    • This method expands the applicability of precise genome manipulation to mammalian somatic cells.
    • ZFN technology offers a powerful approach for gene editing and genetic research.