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Related Experiment Video

Updated: Jun 6, 2026

Plastic Embedding and Sectioning of Xenopus laevis Embryos
14:39

Plastic Embedding and Sectioning of Xenopus laevis Embryos

Published on: April 29, 2007

High-throughput Xenopus laevis immunohistochemistry using agarose sections.

Douglas Blackiston1, Laura N Vandenberg, Michael Levin

  • 1Department of Biology, Tufts University, Medford, MA 02155, USA.

Cold Spring Harbor Protocols
|December 3, 2010
PubMed
Summary

This study presents a rapid method for sectioning Xenopus laevis embryos, improving protein localization analysis in developmental studies. The new protocol offers durable, high-resolution tissue sections for antibody detection without harsh treatments.

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Area of Science:

  • Developmental Biology
  • Regenerative Medicine
  • Xenopus laevis Research

Background:

  • Protein localization in Xenopus laevis embryos is crucial for developmental and regenerative studies.
  • Whole-mount immunohistochemistry has limitations in visualizing internal embryonic tissues due to antibody penetration issues.
  • Traditional microtome sectioning of paraffin-embedded embryos is time-consuming, difficult to orient, and can damage proteins.

Purpose of the Study:

  • To develop a rapid and robust protocol for generating Xenopus laevis embryo sections for immunoreactions.
  • To overcome the limitations of existing methods for internal protein localization analysis.
  • To provide a quick screening process for protein expression in embryonic tissues.

Main Methods:

  • A short protocol for preparing durable Xenopus laevis embryo sections.

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Last Updated: Jun 6, 2026

Plastic Embedding and Sectioning of Xenopus laevis Embryos
14:39

Plastic Embedding and Sectioning of Xenopus laevis Embryos

Published on: April 29, 2007

Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining
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Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

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Published on: April 3, 2013

  • Sections are processed without heating or harsh reagents, preserving protein integrity.
  • Embryos are oriented easily within a transparent block for sectioning.

    • Main Results:

    • The protocol yields robust sections suitable for immunoreactions within two days from sample collection to visualization.
    • Sections can be handled like whole mounts, allowing for fluid aspiration processing.
    • The method enables tandem examination of multiple antibody targets without autofluorescence.

    Conclusions:

    • This new sectioning method offers a rapid, efficient, and versatile approach for protein localization in Xenopus laevis embryos.
    • It overcomes key limitations of whole-mount and traditional sectioning techniques.
    • The protocol is highly beneficial for developmental biology and regenerative medicine research utilizing Xenopus laevis.