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Updated: Jun 6, 2026

12:10
Microinjection of Xenopus Laevis Oocytes
Published on: February 23, 2009
Calibration of the injection volume for microinjection of Xenopus oocytes and embryos
Cold Spring Harbor Protocols
|December 3, 2010
Abstract:
Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) or DNA is a powerful technique for studying development. Before microinjection can be performed, the injection volume must be calibrated carefully. This protocol describes the calibration procedure for a pressure injector.
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