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Related Experiment Videos

Increased matriptase zymogen activation in inflammatory skin disorders.

Cheng-Jueng Chen1, Bai-Yao Wu, Pai-In Tsao

  • 1Dept. of Dermatolog, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.

American Journal of Physiology. Cell Physiology
|December 3, 2010
PubMed
Summary
This summary is machine-generated.

Matriptase, an enzyme crucial for skin barrier function, becomes abnormally activated in many inflammatory skin diseases. This activation may be triggered by factors like acidity and reactive oxygen species.

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Area of Science:

  • Dermatology
  • Biochemistry
  • Protease Biology

Background:

  • Matriptase and its inhibitor HAI-1 are vital for maintaining epidermal barrier integrity.
  • Matriptase activity is precisely controlled in normal skin.
  • Dysregulation of this protease system is suspected in skin pathologies.

Purpose of the Study:

  • To investigate matriptase and HAI-1 levels and activation in various human skin disorders.
  • To determine if matriptase is activated in inflammatory skin conditions.

Main Methods:

  • Examined matriptase and HAI-1 protein distribution in normal epidermis and 23 human skin disorders.
  • Assessed activated matriptase levels in skin tissues.
  • Induced matriptase activation in HaCaT cells using acidic pH and hydrogen peroxide.

Main Results:

  • Matriptase and HAI-1 showed specific localization patterns in normal epidermis.
  • Activated matriptase was significantly increased in keratinocytes of 16 out of 23 skin diseases studied.
  • Acidic pH and H(2)O(2) induced matriptase activation in cell culture.

Conclusions:

  • Inflammation in skin diseases is associated with increased matriptase activation.
  • Tissue acidity and reactive oxygen species may contribute to matriptase activation in certain skin disorders.
  • Matriptase dysregulation is implicated in the pathogenesis of inflammatory skin conditions.