Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preexisting Fundus Findings in a Large Cohort of Young Cynomolgus Macaques (<i>Macaca fascicularis</i>) Bred for Ocular Research: Comparison to Humans.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics·2026
Same author

Spatial Activity Patterning and Topological Defect Transport in Acoustically Energized Active Liquid Crystals.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Origins of Enhanced Ion Transport in Nanostructured Anion-Conducting Polyelectrolytes.

Journal of the American Chemical Society·2026
Same author

The Role of Water Volume Fraction on Water Adsorption in Anion Exchange Membranes.

Macromolecules·2026
Same author

Spontaneous refractive error, ocular biometry and age related lens changes in a population of geriatric rhesus macaques.

Scientific reports·2025
Same author

Topical Application of a Novel Harderian-Derived Nonpolar Lipid Prolongs Tear Film Breakup Time in Normal Beagle Dogs.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics·2025

Related Experiment Video

Updated: Jun 6, 2026

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

The applications of atomic force microscopy to vision science.

Julie A Last1, Paul Russell, Paul F Nealey

  • 1Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Investigative Ophthalmology & Visual Science
|December 3, 2010
PubMed
Summary

Atomic Force Microscopy (AFM) offers advanced imaging and mechanical property analysis for biological materials. This review highlights its potential applications and encourages its adoption in vision science research.

More Related Videos

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Related Experiment Videos

Last Updated: Jun 6, 2026

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Area of Science:

  • Ophthalmology and Vision Science
  • Biophysics
  • Materials Science

Background:

  • Atomic Force Microscopy (AFM) is a powerful tool for high-resolution imaging and mechanical property analysis of biological samples.
  • While widely used in materials and biological sciences, AFM applications in vision science remain underexplored.
  • AFM's ability to probe soft biological materials in native environments presents unique opportunities for ocular research.

Purpose of the Study:

  • To review the operational principles of Atomic Force Microscopy (AFM).
  • To survey the existing applications of AFM within the field of vision science.
  • To encourage the integration of AFM into vision science research methodologies.

Main Methods:

  • Review of existing literature on AFM operation and applications.
  • Analysis of AFM's capabilities for imaging and mechanical probing of biological tissues.
  • Case studies of AFM implementation in vision science research.

Main Results:

  • AFM enables detailed topographic imaging of biological surfaces.
  • AFM can quantify mechanical properties like elastic modulus of cells and extracellular matrices.
  • AFM allows investigation of receptor-ligand interactions at the cellular level.

Conclusions:

  • AFM technology is well-suited for investigating the micro- and nanostructure of ocular tissues.
  • The mechanical properties of the eye, probed by AFM, are crucial for understanding various vision disorders.
  • This review aims to bridge the gap and promote AFM adoption in vision science for novel discoveries.