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Bacillus amyloliquefaciens alpha-amylase signal sequence fused in frame with human proinsulin is properly processed
A A Novikov1, S I Borukhov, Strongin AYa
1Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
Biochemical and Biophysical Research Communications
|May 31, 1990
Abstract:
The plasmid pBINS1, containing the promoter, SD and leader peptide sequences of Bacillus amyloliquefaciens alpha-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of protease-deficient Bacillus subtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.