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Updated: Jun 6, 2026
15:22
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Merely two mutations switch a DNA-hydrolyzing deoxyribozyme from heterobimetallic (Zn2+/Mn2+) to monometallic
Ying Xiao1, Emily C Allen, Scott K Silverman
1Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
Chemical Communications (Cambridge, England)
|December 3, 2010
Abstract:
A deoxyribozyme that hydrolyzes DNA phosphodiester linkages with a requirement for both Zn(2+) and Mn(2+) is switched by only two nucleotide mutations to require Zn(2+) alone, demonstrating that DNA-catalyzed DNA hydrolysis can be achieved using only one metal ion cofactor.
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