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Validation of RNAi by real time PCR

Knud Josefsen1, Ying C Lee

  • 1The Bartholin Institute, Copenhagen University Hospital, Copenhagen, Denmark. knud@eln.dk

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2010
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...