Related Experiment Video

Updated: Jun 6, 2026

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
07:39

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

TGFβ-dependent gene expression shows that senescence correlates with abortive differentiation along several lineages

Judith Müller1, Birgit Samans, Jan van Riggelen

  • 1Theodor Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany.

Cell Cycle (Georgetown, Tex.)
|December 4, 2010
PubMed

Insights

Turning off Myc expression in lymphoma-prone mice triggers TGFβ-dependent cellular differentiation, revealing insights into senescence and T-cell lineage development.

Area of Science:

  • * Molecular biology
  • * Cancer research
  • * Immunology

Background:

  • * Deregulated Myc expression drives lymphoma in mice.
  • * Transforming growth factor beta (TGFβ)-dependent senescence limits lymphoma development.
  • * Continuous high Myc levels are needed to counteract senescence.

Purpose of the Study:

  • * To comprehensively analyze TGFβ-dependent gene expression changes after Myc transgene inactivation.
  • * To elucidate the biological processes underlying TGFβ-dependent senescence.

Main Methods:

  • * Gene expression profiling in mice with inducible Myc transgenes.
  • * Analysis of TGFβ-dependent and -independent gene regulation.
  • * Comparison of gene expression patterns with Polycomb repressive complex targets.

Main Results:

  • * Myc-induced genes decrease independently of TGFβ upon Myc inactivation.
  • * TGFβ upregulates T-cell lineage-specific genes when Myc is off.
  • * Myc-repressed genes show overlap with Polycomb targets in stem cells.

Conclusions:

  • * TGFβ-dependent senescence involves gene expression patterns of abortive cellular differentiation.
  • * Myc inactivation leads to TGFβ-mediated upregulation of lineage-specific genes.
  • * Findings link senescence, differentiation, and Polycomb regulation in lymphoma.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...