Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial calcium regulates lipid metabolism by modulating tethering of mitochondria to lipid droplets.

The EMBO journal·2026
Same author

Targeting lysosomal pH restores mitochondrial quality control in GBA1-mutant Parkinson's disease.

Translational neurodegeneration·2026
Same author

Adolescent obesity and risk of serious chronic morbidity and mortality in young adulthood: a systematic review of cohorts from national registries.

Cardiovascular diabetology·2026
Same author

A Tale of Three CTs: A Hip Fracture in a Patient with Down Syndrome Uncovers an Endocrinologic Iceberg.

The Israel Medical Association journal : IMAJ·2026
Same author

Drp1 regulates mitochondrial health and controls skeletal muscle mass through the Erk1/2-Nur77 pathway.

Science advances·2026
Same author

Obesity in Late Adolescence and Healthcare Costs in Young to Mid-Adulthood.

Diabetes, obesity & metabolism·2026
Same journal

Inflammation Impairs Poststroke Recovery by Disrupting Iron Homeostasis in Brain.

Antioxidants & redox signaling·2026
Same journal

Histone Lactylation Links Glycolysis to Ferroptosis in Diabetic Cataract.

Antioxidants & redox signaling·2026
Same journal

Insights into the Multifaceted Roles of 3-Mercaptopyruvate Sulfurtransferase in Liver Diseases.

Antioxidants & redox signaling·2026
Same journal

NINJ1 Aggravates Doxorubicin-Induced Cardiotoxicity by Suppressing AMPK-Mediated HIF-1α Deubiquitination.

Antioxidants & redox signaling·2026
Same journal

<i>Corrigendum to:</i> Suppression of Cardiac Autophagy by Hyperinsulinemia in Insulin Receptor-Deficient Hearts Is Mediated by Insulin-Like Growth Factor Receptor Signaling.

Antioxidants & redox signaling·2026
Same journal

Sleeve Gastrectomy Is Associated with Improved Systemic Redox Homeostasis in T2DM Through Ghrelin-GHSR Attenuation, POMC Neuronal Modulation, and CD4<sup>+</sup> T Cell Metabolic Reprogramming.

Antioxidants & redox signaling·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

The interplay between mitochondrial dynamics and mitophagy.

Gilad Twig1, Orian S Shirihai

  • 1Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA.

Antioxidants & Redox Signaling
|December 7, 2010
PubMed
Summary
This summary is machine-generated.

Mitochondrial dynamics, including fusion and fission, interact with mitophagy to maintain cellular health. These processes coordinate to manage impaired mitochondria, ensuring cellular homeostasis.

More Related Videos

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs
08:15

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

Related Experiment Videos

Last Updated: Jun 6, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs
08:15

Understanding the Changes in Mitochondrial Morphology through Dynamic and Three-dimensional Fluorescence Micrographs

Published on: August 15, 2025

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

Area of Science:

  • Cell Biology
  • Mitochondrial Biology
  • Autophagy

Background:

  • Mitochondrial homeostasis is crucial for cellular function.
  • Mitochondrial dynamics (fusion and fission) and mitophagy are key regulatory processes.
  • The interplay between these processes is not fully understood.

Purpose of the Study:

  • To characterize the life cycle of individual mitochondria.
  • To investigate the interaction between mitochondrial dynamics and mitophagy.
  • To explore their roles in different tissues and disease models.

Main Methods:

  • Morphological characterization of mitochondria.
  • Bioenergetic analysis of mitochondrial function.
  • Analysis of mitophagy pathways.

Main Results:

  • Mitochondrial fission can generate impaired mitochondria targeted for mitophagy.
  • Mitochondrial fusion may prevent the removal of impaired components.
  • Fusion and mitophagy exhibit inverse dependency on membrane potential, acting complementarily.

Conclusions:

  • Mitochondrial dynamics and mitophagy are coordinated processes for maintaining mitochondrial quality control.
  • Their interplay is essential for cellular homeostasis under various conditions.
  • Understanding this relationship is vital for studying mitochondrial diseases.