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Related Experiment Videos

Aflatoxin B1 in compound-feed reference materials: an intercomparison of methods.

H P Van Egmond1, P J Wagstaffe

  • 1Laboratory for Residue Analysis, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

Food Additives and Contaminants
|March 1, 1990
PubMed
Summary

This study validated methods for detecting aflatoxin B1 in animal feed, crucial for analytical quality control. Multiple laboratory methods showed good agreement, supporting the development of reliable compound-feed reference materials.

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DON calibrant: Towards the production of certified B-trichothecene calibrants.

Mycotoxin research·2013

Area of Science:

  • Food Safety
  • Analytical Chemistry
  • Mycotoxin Analysis

Background:

  • Ensuring analytical quality control for mycotoxin detection in compound feed is essential.
  • The BCR initiated a project to develop compound-feed reference materials for aflatoxin B1 analysis.
  • Aflatoxin B1 poses a significant risk to animal health and food safety.

Purpose of the Study:

  • To describe the first phase of a project focused on intercomparing methods for aflatoxin B1 determination in compound feedstuffs.
  • To evaluate the performance of various analytical methods at a target concentration of approximately 15 µg/kg.
  • To establish a basis for the development of certified reference materials for aflatoxin B1.

Main Methods:

  • An interlaboratory study involving 24 European laboratories analyzing two pairs of compounded feedstuffs (contaminated and near-blank).

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  • Utilized diverse extraction solvents (chloroform, methanol-water, acetonitrile-water), clean-up techniques (TLC, disposable cartridges, immunoaffinity columns), and determinative steps (TLC, HPLC, ELISA).
  • Assessed performance characteristics and determined aflatoxin B1 content.

    • Main Results:

    • Recoveries for aflatoxin B1 ranged from approximately 70% to 110%.
    • Coefficients of variation for the contaminated samples were 16% and 19%.
    • Multiple analytical methods, with variations in extraction, clean-up, and detection, yielded results in close agreement.

    Conclusions:

    • The intercomparison demonstrated that various analytical approaches are suitable for determining aflatoxin B1 in compound feedstuffs.
    • A sound basis exists for establishing reference values for compound feed reference materials.
    • This work supports the development of reliable quality control measures for mycotoxin analysis in animal feed.