Related Experiment Video
Updated: Jun 6, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
Method development for β-glucogallin and gallic acid analysis: application to urinary pharmacokinetic studies
Jody K Takemoto1, Neal M Davies
1College of Pharmacy, Department of Pharmaceutical Sciences and Graduate Program in Pharmaceutical Sciences, Washington State University, Pullman, WA 99164-6534, USA.
Abstract:
A reversed phase high-pressure liquid chromatographic electrospray tandem mass spectrometry method was developed for the simultaneous detection and analysis of β-glucogallin and gallic acid. This method used a C(18) column with ultraviolet detection at 285 nm, ionization in the negative ion mode for β-glucogallin and gallic acid, and in the positive ion mode for the internal standard 3-morpholin-4-ylpropane-1-sulfonic acid. Mobile phase consisted of a mixture of water, methanol, and formic acid at a flow rate of 0.7 mL/min. This method was validated over a concentration range of 1-100 μg/mL in rat urine.
More Related Videos
Related Concept Videos
Measurement of Bioavailability: Pharmacokinetic Methods
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation
On...
Phase II Reactions: Glucuronidation
Therapeutic Drug Monitoring: Drug Analysis Methods
One-Compartment Open Model: Urinary Excretion Data and Determination of k
Measurement of Bioavailability: Pharmacodynamic Methods

