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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

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Recent progress in microchip electrophoresis-mass spectrometry.

Fumihiko Kitagawa¹, Koji Otsuka

¹Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. fkitagawa@anchem.mc.kyoto-u.ac.jp

Journal of Pharmaceutical and Biomedical Analysis

|December 7, 2010

Summary

This review covers advancements in microchip electrophoresis-mass spectrometry (MCE-MS) since 1997. It focuses on ionization interfaces, particularly electrospray ionization (ESI) and laser desorption ionization (LDI), and their applications in analyzing biogenic compounds.

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Area of Science:

Analytical Chemistry
Separation Science
Mass Spectrometry

Background:

Microchip electrophoresis-mass spectrometry (MCE-MS) is a powerful analytical technique.
Development of sensitive and efficient ionization interfaces is crucial for MCE-MS.
Electrospray ionization (ESI) and laser desorption ionization (LDI) are key interface technologies.

Purpose of the Study:

To review methodological and instrumental developments in MCE-MS since 1997.
To present the characteristics of ESI and LDI interfaces in MCE-MS.
To provide a comprehensive overview of MCE-MS applications.

Main Methods:

Review of literature on MCE-MS developments from 1997 onwards.
Analysis of ionization interface designs, focusing on ESI and LDI.
Compilation of application data for MCE-MS.

Main Results:

Electrospray ionization (ESI) is the most commonly used interface due to its simple structure suitable for microchips.
Laser desorption ionization (LDI) interfaces have also been developed for MCE-MS, though less frequently published.
MCE-MS applications primarily focus on the analysis of biogenic compounds, including bioactive amines, peptides, and proteins.

Conclusions:

Ionization interface development is critical for enhancing MCE-MS sensitivity and separation efficiency.
Both ESI and LDI interfaces offer distinct advantages for MCE-MS applications.
MCE-MS is a versatile technique for analyzing complex biological samples.