Related Experiment Video
Updated: Jun 6, 2026

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
Published on: June 23, 2016
Visual observation of avidin-biotin affinity by fluorescent G4.5 poly(amidoamine) dendrimer
Govindachetty Saravanan1, Kenji Daigo, Toyoko Imae
1Graduate School of Science and Technology, Keio University, Hiyoshi, Yokohama 223-8522, Japan.
Abstract:
An air-treated G4.5 poly(amidoamine) (PAMAM) dendrimer displayed the enhanced fluorescence enough to be utilized as a fluorescence marker to visualize avidin-biotin affinity: On a fluorescence microscopic image, the avidin labeled by a fluorescent G4.5 PAMAM dendrimer was observed to be selectively bound on the biotin pattern that was prepared by amide-bonding of biotin on a carboxylic acid-terminated self-assembled monolayer and in turn by UV-irradiation with a photomask on the monolayer.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

