Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determination of bioequivalence between generic and reference drugs using laser-induced breakdown spectroscopy.

Analytica chimica acta·2024
Same author

The Ultratrace Determination of Fluoroquinolones in River Water Samples by an Online Solid-Phase Extraction Method Using a Molecularly Imprinted Polymer as a Selective Sorbent.

Molecules (Basel, Switzerland)·2022
Same author

Analysis of alternariol and alternariol monomethyl ether in foodstuffs by molecularly imprinted solid-phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry.

Food chemistry·2017
Same author

Multiresidue analysis of cephalosporin antibiotics in bovine milk based on molecularly imprinted polymer extraction followed by liquid chromatography-tandem mass spectrometry.

Journal of chromatography. A·2016
Same author

Light-emitting-diodes based on ordered InGaN nanocolumns emitting in the blue, green and yellow spectral range.

Nanotechnology·2014
Same author

Multiresidue determination of fluoroquinolone antimicrobials in baby foods by liquid chromatography.

Food chemistry·2014

Related Experiment Video

Updated: Jun 6, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Molecularly imprinted polymer diffraction grating as label-free optical bio(mimetic)sensor.

C A Barrios1, C Zhenhe, F Navarro-Villoslada

  • 1Instituto de Sistemas Optoelectrónicos y Microtecnología, ETSI Telecomunicación, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. carlos.angulo.barrios@upm.es

Biosensors & Bioelectronics
|December 7, 2010
PubMed
Summary
This summary is machine-generated.

This study presents novel molecularly imprinted polymer (MIP) diffraction gratings for label-free antibiotic sensing. These bio(mimetic) sensors demonstrate high specificity in detecting enrofloxacin through optical diffraction changes.

More Related Videos

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Related Experiment Videos

Last Updated: Jun 6, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Label-free biosensors are crucial for rapid detection of analytes.
  • Molecularly imprinted polymers (MIPs) offer tailored recognition sites for specific molecules.
  • Diffraction gratings provide a sensitive optical transduction mechanism.

Purpose of the Study:

  • To fabricate and evaluate micropatterned MIP transmissive 2D diffraction gratings (DGs) as label-free antibiotic biosensors.
  • To demonstrate the specificity and sensitivity of MIP DGs for antibiotic detection.
  • To explore the use of optical diffraction efficiency changes as a sensor response.

Main Methods:

  • Fabrication of MIP 2D-DGs using microtransfer molding with SiO(2)/Si molds.
  • Morphological characterization using optical and atomic force microscopy.
  • Detection of enrofloxacin by measuring changes in diffraction efficiency upon analyte immersion.

Main Results:

  • MIP 2D-DGs successfully produced 2D optical diffraction patterns.
  • Significant variations in diffraction efficiency were observed upon exposure to enrofloxacin.
  • High specificity was confirmed through control experiments with non-target analytes and non-imprinted polymers.

Conclusions:

  • Micropatterned MIP 2D-DGs function as effective label-free biosensors for antibiotic detection.
  • The sensor exhibits high selectivity for the target antibiotic, enrofloxacin.
  • Optical diffraction efficiency changes serve as a reliable indicator for label-free recognition.