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SAINT: probabilistic scoring of affinity purification-mass spectrometry data
Hyungwon Choi1, Brett Larsen, Zhen-Yuan Lin
1Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA.
Abstract:
We present 'significance analysis of interactome' (SAINT), a computational tool that assigns confidence scores to protein-protein interaction data generated using affinity purification-mass spectrometry (AP-MS). The method uses label-free quantitative data and constructs separate distributions for true and false interactions to derive the probability of a bona fide protein-protein interaction. We show that SAINT is applicable to data of different scales and protein connectivity and allows transparent analysis of AP-MS data.
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