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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 6, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Shotgun glycomics: a microarray strategy for functional glycomics.

Xuezheng Song1, Yi Lasanajak, Baoyun Xia

  • 1Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA.

Nature Methods
|December 7, 2010
PubMed
Summary
This summary is machine-generated.

Shotgun glycomics enables detailed characterization of cellular glycans, specifically glycosphingolipids (GSLs). This method identifies functional glycans and their interactions with proteins, advancing glycomics research.

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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Area of Science:

  • Glycobiology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Characterizing complex glycomes and identifying functional glycans as ligands for glycan-binding proteins (GBPs) are significant challenges in glycomics.
  • Glycosphingolipids (GSLs) are a particularly challenging class of glycoconjugates to study, despite their recognition by toxins, antibodies, and GBPs.

Purpose of the Study:

  • To develop a general strategy, termed shotgun glycomics, for characterizing GSLs and identifying functionally important glycans.
  • To create GSL shotgun microarrays for interrogating biological interactions and facilitating structural analysis.

Main Methods:

  • Extraction and derivatization of GSLs from cells using a heterobifunctional fluorescent tag.
  • Separation of fluorescent GSLs by multidimensional chromatography and quantification.
  • Immobilization of fluorescent GSLs onto glass slides to create GSL shotgun microarrays.
  • Interrogation of microarrays with cholera toxin, antibodies, and patient sera, followed by mass spectrometry.

Main Results:

  • Successful creation and application of GSL shotgun microarrays.
  • Identification of biologically relevant GSLs through interactions with specific probes (cholera toxin, antibodies, Lyme disease sera).
  • Characterization of identified GSLs using mass spectrometry.

Conclusions:

  • Shotgun glycomics is a viable approach for accessing and analyzing the complex glycomes of animal cells.
  • This strategy allows for focused structural analysis on functionally important glycans, advancing the understanding of glycan roles in biological systems.