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Updated: Jun 6, 2026
Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Comparison of two PCR systems for the rapid detection of Leptospira spp. from kidney tissue
Anne Mayer-Scholl1, Angelika Draeger, Enno Luge
1Federal Institute for Risk Assessment (BfR), Berlin, Germany. anne.mayer-scholl@bfr.bund.de
Abstract:
In this study we compared two routine PCR systems for the detection of Leptospira spp. and assessed their performance when directly applied to kidney samples from small mammals. Although the kappa value of 0.9 indicated a high level of agreement between the tests, the outer membrane lipoprotein gene lipl32 based PCR was more robust and showed a higher number of positive kidney samples.
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