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A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

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Imaging visible light with Medipix2.

Aaron Mac Raighne¹, Colin Brownlee, Ulrike Gebert

¹Department of Physics and Astronomy, University of Glasgow, Glasgow G12 8QQ, United Kingdom.

The Review of Scientific Instruments

|December 8, 2010

Summary

This summary is machine-generated.

This study presents a novel high-speed optical imaging detector using the Medipix2 chip for single-photon counting. The device achieves over 3000 frames/s, enabling advanced imaging applications.

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Area of Science:

Photonics and Detector Technology
High-Speed Optical Imaging
Semiconductor Device Physics

Background:

Existing optical imaging detectors lack the high speed required for certain applications.
Single-photon counting for X-ray detection is feasible using Medipix2 and silicon photodiodes.
There is a specific need for high-speed, single-photon counting capabilities in optical imaging.

Purpose of the Study:

To develop and characterize a novel optical imaging detector utilizing the Medipix2 chip.
To evaluate the high-speed imaging performance of the fabricated detector.
To investigate the impact of ion feedback on the imaging properties of the device.

Main Methods:

Fabrication of a detector device incorporating the Medipix2 chip and pixelated silicon photodiodes.
High-speed imaging experiments conducted on the fabricated 256x256 pixel matrix detector.
Modulation Transfer Function (MTF) measurements to quantify imaging performance.
Analysis of ion feedback effects on detector image quality.

Main Results:

The fabricated detector achieves imaging speeds exceeding 3000 frames per second.
The detector operates over a 256x256 pixel matrix.
Modulation Transfer Function (MTF) was measured to assess imaging performance.
Ion feedback was identified as a factor potentially degrading imaging properties.

Conclusions:

The developed Medipix2-based detector offers high-speed, single-photon counting optical imaging capabilities.
The device demonstrates potential for applications requiring rapid image acquisition.
Further investigation into mitigating ion feedback is recommended to optimize imaging performance.