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Updated: Jun 6, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Restructuring proteomics through verification.

Emily Boja¹, Robert Rivers, Christopher Kinsinger

¹Office of Cancer Clinical Proteomics Research, Center for Strategic Scientific Initiative, National Cancer Institute, NIH, 31 Center Drive, MS 2590, Bethesda, MD 20892, USA.

Biomarkers in Medicine

|December 8, 2010

Summary

This summary is machine-generated.

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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Proteomics research faces challenges in identifying cancer biomarkers. This article outlines a reliable pipeline for protein biomarker development, emphasizing verification using multiplex targeted proteomics for clinical applications.

Area of Science:

Biochemistry
Cell Biology
Biomarker Discovery

Background:

Proteomics technologies offer powerful tools for biological research.
Translational cancer research faces challenges in biomarker discovery using proteomics.
Skepticism exists regarding the success of proteomics in identifying clinical cancer biomarkers.

Purpose of the Study:

To highlight the development of a reliable and efficient protein biomarker pipeline.
To discuss the translation of proteomics research into clinical applications for cancer.
To evaluate promising technologies for biomarker verification.

Main Methods:

Review of proteomics technologies for biomarker discovery.
Description of a three-step biomarker development pipeline: discovery, verification, and qualification.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Discussion of multiple reaction monitoring mass spectrometry for high-throughput protein biomarker measurement.

Main Results:

The National Cancer Institute is actively facilitating the translation of proteomics.
A structured pipeline is proposed to enhance the reliability and efficiency of biomarker development.
Multiple reaction monitoring mass spectrometry shows promise for preclinical biomarker verification.

Conclusions:

Developing a robust biomarker pipeline is crucial for clinical proteomics in cancer research.
Multiplex targeted proteomics platforms like multiple reaction monitoring mass spectrometry are key for efficient verification.
Continued efforts are needed to overcome skepticism and translate proteomics discoveries into clinical tools.