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Updated: Aug 28, 2026

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
Published on: February 12, 2014
Binding and internalization of ricin labelled with fluorescein isothiocyanate
A Bellelli1, R Ippoliti, M Brunori
1Dipartimento di Scienze Biochimiche e Centro di Biologia Molecolare del C.N.R., Universita di Roma La Sapienza, Italy.
Abstract:
The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photobleaching recovery, is D = 1 - 2 x 10(-10) cm2/s. Fluorescence microscopy provides preliminary evidence for secondary endosomes in the cytoplasm.
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