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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Separating nanoparticles from microemulsions.

Muhammad Faizan Nazar¹, Olesya Myakonkaya, Syed Sakhawat Shah

¹Department of Chemistry, Quaid-i-Azam University, Islamabad, Pakistan. faizan_qau@yahoo.com

Journal of Colloid and Interface Science

|December 8, 2010

Summary

A new method uses water-in-oil microemulsions to create and separate gold nanoparticles (Au-NPs) and other nanoparticles. This cost-effective process recovers over 90% of Au-NPs and preserves their structure.

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Area of Science:

Materials Science
Colloid and Surface Chemistry

Background:

Water-in-oil microemulsions (w/o μEs) offer unique reaction media for nanoparticle synthesis.
Dispersing pre-synthesized nanoparticles (Au-NPs, Pd-NPs, SiO(2)-NPs) in microemulsions presents separation challenges.

Purpose of the Study:

To develop a general, low-energy method for recovering and separating various nanoparticles from microemulsion systems.
To demonstrate the effectiveness of tuning microemulsion phase behavior for nanoparticle purification.

Main Methods:

Utilizing water-in-oil microemulsions (w/o μEs) stabilized by cetyltrimethylammonium chloride (CTACl) for nanoparticle generation and dispersion.
Inducing liquid-liquid phase transitions by adding water to separate nanoparticles into an oil-rich phase.
Employing UV-vis and (1)H NMR spectroscopy for process monitoring and recovery quantification; gravimetry for silica NPs; TEM for structural analysis.

Main Results:

A clean liquid-liquid phase separation was achieved, partitioning nanoparticles into an upper oil-rich layer.
High recovery efficiencies were demonstrated: ~90% for microemulsion-prepared Au-NPs, ~98% for Au-MES-NPs, 92% for Pd-MES-NPs, and ~84% for SiO(2)-NPs.
Transmission Electron Microscopy (TEM) confirmed that nanoparticle size and shape were preserved post-separation.

Conclusions:

The developed method provides a cost-effective, isothermal, and general approach for purifying diverse inorganic nanoparticles from microemulsion systems.
This purification route avoids additional organic solvents and utilizes inexpensive, commercially available components.
The technique facilitates efficient recovery and recycling of nanoparticles, enhancing their practical applicability.