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Related Experiment Video

Updated: Jun 6, 2026

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

THE HUPO Human Plasma Proteome Project.

Gilbert S Omenn1

  • 1Internal Medicine, Human Genetics, Public Health and Center for Computational Medicine and Biology, University of Michigan, Ann Arbor, MI, USA. gomenn@umich.edu.

Proteomics. Clinical Applications
|December 8, 2010
PubMed
Summary

The Human Plasma Proteome Project (PPP) created a core dataset of 3020 human proteins, fostering collaborative research. This valuable resource aids ongoing studies in human health and disease proteomics.

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Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Area of Science:

  • Proteomics
  • Human Biology
  • Biochemistry

Background:

  • The Human Proteomics Organization (HUPO) initiated the Human Plasma Proteome Project (PPP) to characterize the human proteome.
  • Characterizing the human proteome is crucial for understanding health and disease states.

Purpose of the Study:

  • To establish a comprehensive dataset of human plasma proteins.
  • To facilitate collaborative international research in proteomics.
  • To provide a foundation for future proteomic analyses.

Main Methods:

  • International collaboration involving 55 laboratories.
  • Data generation and curation for a core protein dataset.
  • Independent analysis of raw spectral data.

Main Results:

  • Generated a Core Dataset of 3020 proteins identified by two or more peptides.
  • Dataset is publicly accessible via EBI/PRIDE, ISB/PeptideAtlas, and University of Michigan.
  • Provided protein annotation and correlation with immunoassay concentrations.

Conclusions:

  • The PPP pilot phase successfully created a valuable, accessible resource for the scientific community.
  • Future PPP phases will focus on standardized procedures, new technologies, and advanced informatics.
  • The project highlights the success of large-scale, collaborative efforts in human proteomics.

Related Experiment Videos

Last Updated: Jun 6, 2026

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014