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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 6, 2026

Synthetic Antigen Controls for Immunohistochemistry
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Synthetic Antigen Controls for Immunohistochemistry

Published on: August 23, 2021

Antibody-based Proteomics: From bench to bedside.

Sophie Dahan1, Eric Chevet, Jian-Feng Liu

  • 1HyperOmics Farma Inc., Pierrefonds, QC, Canada.

Proteomics. Clinical Applications
|December 8, 2010
PubMed
Summary
This summary is machine-generated.

Antibodies have evolved from biomarkers to powerful therapeutics. This review explores antibody-based proteomics for clinical applications, detailing methods for marker identification, antibody production, and validation for bench and bedside use.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

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Last Updated: Jun 6, 2026

Synthetic Antigen Controls for Immunohistochemistry
09:30

Synthetic Antigen Controls for Immunohistochemistry

Published on: August 23, 2021

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Immunology
  • Proteomics
  • Biotechnology

Background:

  • Antibodies have transitioned from disease biomarkers to potent therapeutic agents over 75 years.
  • The identification of monoclonal antibodies (mAbs) and advancements in immunological tools have accelerated this evolution.
  • Antibody-based proteomics offers a powerful approach to analyze cellular and tissue proteomes.

Purpose of the Study:

  • To review the application of antibodies in assessing cellular and tissue proteomes.
  • To discuss the integration of large-scale datasets with clinical data for marker identification.
  • To compare antibody production methodologies and validation tools for clinical relevance.

Main Methods:

  • Integration of high-throughput proteomics and transcriptomics data with literature and clinical data.
  • Literature review and expert experience to compare in vivo and in vitro antibody production techniques.
  • Description of available validation tools for antibody-based approaches.

Main Results:

  • Antibody-based proteomics requires specific analytical and technological pipelines.
  • Methodologies for producing specific antibodies (in vivo and in vitro) have been compared.
  • Validation tools and the use of molecular signatures for clinical applications are presented.

Conclusions:

  • Antibodies are crucial tools for proteomic assessment with significant clinical potential.
  • Effective antibody development and validation are essential for translating research findings to clinical practice.
  • Antibody-based molecular signatures are emerging for both research and clinical settings.