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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: Jun 6, 2026

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Published on: January 13, 2023

In vivo secretome sampling technology for proteomics.

Chun-Ming Huang¹

¹Division of Dermatology, Department of Medicine, San Diego, CA, USA; Moores Cancer Center, University of California, San Diego, CA, USA; La Jolla Institute for Molecular Medicine, San Diego, CA, USA; VA San Diego Healthcare Center, San Diego, CA, USA. chunming@ucsd.edu.

Proteomics. Clinical Applications

|December 8, 2010

Summary

Capillary ultrafiltration (CUF) probes offer a novel in vivo sampling technique for collecting pure secretomes from tissue microenvironments. This method provides significant advantages for systems biology research and potential clinical applications.

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Area of Science:

Biochemistry
Systems Biology
Biomarker Discovery

Background:

The secretome, comprising secreted proteins, is crucial for cell-cell and microbe-cell communication.
Identifying secretome components offers valuable biomarkers and therapeutic targets.
Current methods for in vivo secretome sampling have limitations.

Purpose of the Study:

To introduce and review the capillary ultrafiltration (CUF) probe technique for in vivo secretome collection.
To highlight the benefits and adaptability of CUF probes for various research and clinical applications.

Main Methods:

Development and application of capillary ultrafiltration (CUF) probes for continuous in vivo secretome sampling.
Adaptation of CUF probes for diverse in vivo models and integration with protein detection systems like mass spectrometry.

Main Results:

CUF probes enable pure secretome collection directly from tissue microenvironments.
The technique offers advantages over existing sampling methods in terms of simplicity and dynamic sampling.
CUF probes are compatible with various in vivo models and analytical platforms.

Conclusions:

CUF probes represent a significant advancement in in vivo secretome analysis.
The technology holds promise for improved systems biology research, biomarker discovery, and future clinical applications.
Prospective properties like pathogen clearance and online mass spectrometric coupling enhance its clinical utility.