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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
Proteoglycans01:05

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...

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Related Experiment Video

Updated: Jun 6, 2026

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

Vascular proteomics.

Fernando Vivanco1, Sebastian Mas, Veronica M Darde

  • 1Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain; Department of Biochemistry and Molecular Biology I, Universidad Complutense, Proteomic Unit, Madrid, Spain. fvivanco@fjd.es.

Proteomics. Clinical Applications
|December 8, 2010
PubMed
Summary
This summary is machine-generated.

Proteomics offers new insights into acute coronary syndromes (ACS) by identifying novel protein biomarkers in blood and atherosclerotic lesions. This approach aids in earlier disease detection and monitoring therapeutic responses for cardiovascular diseases (CVD).

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Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
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Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

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Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Cardiovascular Proteomics
  • Molecular Pathology of Atherosclerosis

Background:

  • Acute coronary syndromes (ACS) require molecular and cellular characterization for better understanding.
  • Proteomic technologies have advanced the study of protein expression changes in cardiovascular diseases (CVD).
  • Blood-based biomarker discovery is a promising avenue for CVD detection and management.

Purpose of the Study:

  • To review proteomic strategies for studying atherosclerosis.
  • To identify novel protein biomarkers for ACS and CVD.
  • To explore the potential of proteomics in early disease detection and therapy monitoring.

Main Methods:

  • Systematic evaluation of protein expression changes using proteomic technologies.
  • Analysis of atherosclerotic lesions and plasma samples.
  • Mass spectrometry (MS)-based imaging of atheroma plaque to visualize lipid distribution.
  • Proteomic profiling of circulating cells and individual cells within plaques.

Main Results:

  • Identification of differentially expressed proteins in atherosclerotic lesions (e.g., HSP-27, Cathepsin D) as potential biomarkers.
  • Generation of 2-D lipid images from atheroma plaque using MS-Imaging.
  • Discovery of novel plasma biomarkers (e.g., amyloid A1α, transthyretin).
  • Characterization of protein profiles in monocytes from ACS patients and plaque-associated cells.

Conclusions:

  • Proteomics provides novel insights into vascular pathophysiology and ACS.
  • Identified proteins and imaging techniques offer potential for improved CVD diagnostics and therapeutics.
  • Blood-based biomarkers and cellular analysis contribute to a deeper understanding of atherosclerosis.