Related Experiment Video
Updated: Jan 18, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Oxidation of proteins: Basic principles and perspectives for blood proteomics
Stefano Barelli1, Giorgia Canellini, Lynne Thadikkaran
1Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
Protein oxidation causes various modifications, impacting normal functions and defense mechanisms. Blood proteomics requires redox strategies to analyze these changes in response to oxidative stress.
Area of Science:
- Biochemistry
- Proteomics
- Oxidative Stress
Background:
- Protein oxidation encompasses diverse modifications, including backbone cleavage, crosslinking, and side chain alterations.
- Oxidation is integral to cellular regulation, defense against oxidative stress, and pathological processes when overwhelmed.
- Blood's constant exposure to reactive oxygen and nitrogen species necessitates redox proteomic approaches.
Purpose of the Study:
- To review the biochemical underpinnings of protein oxidation.
- To survey proteomic methodologies for analyzing redox modifications.
- To highlight blood component responses to oxidative stress.
Main Methods:
- Literature review of protein oxidation biochemistry.
- Analysis of proteomic techniques for redox-sensitive modifications.
- Compilation of data on physiological and in vitro oxidative stress responses in blood.
Main Results:
- Detailed biochemical mechanisms of protein oxidation are presented.
- Various proteomic strategies for detecting redox modifications are discussed.
- Examples of blood component responses to oxidative stress are highlighted.
Conclusions:
- Redox proteomics is essential for understanding blood components under oxidative stress.
- The review provides a foundation for studying oxidative modifications in blood.
- Further research into blood redox proteomics can elucidate disease mechanisms.
More Related Videos
07:38Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes
Published on: September 1, 2020
09:33Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
Related Concept Videos
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Radical Autoxidation
Peroxisomes
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Overview of Protein Metabolism
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...