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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Proteomic analysis for human urinary proteins associated with arsenic intoxication
Lia-Beng Tan1, Kow-Tong Chen, Yu-Chang Tyan
1Department of Urology, St. Joseph Hospital, Yinlin County, Taiwan; Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Proteomics. Clinical Applications
|December 8, 2010
Summary
Chronic arsenic exposure from well water is linked to bladder cancer. Researchers identified three specific urinary proteins—ADAM, calpain9, and ring finger protein 20—as potential biomarkers for arsenic-associated bladder transitional cell carcinoma (TCC).
Area of Science:
- Environmental Health
- Oncology
- Proteomics
Background:
- Arsenic contamination in drinking water, particularly artesian well water, is a global environmental concern.
- High arsenic levels are associated with genitourinary cancers, notably bladder transitional cell carcinoma (TCC).
- Identifying specific biomarkers for arsenic-induced bladder TCC is crucial for early diagnosis and understanding disease mechanisms.
Purpose of the Study:
- To identify specific urinary protein biomarkers indicative of bladder TCC associated with chronic arsenic exposure.
- To investigate proteomic alterations in urine samples from patients with and without arsenic-associated bladder TCC.
Main Methods:
- Systematic examination of urinary proteomes using High-Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MS/MS).
- Analysis of urine specimens from patients with arsenic-associated TCC, non-arsenic-associated TCC, and matched non-cancer urological controls.
- Comparative proteomic profiling to identify proteins with significantly altered levels in response to arsenic exposure.
Main Results:
- Three urinary proteins showed significantly altered levels in patients with chronic arsenic exposure: a disintegrin and metalloprotease (ADAM) protein, calpain9, and ring finger protein 20.
- HPLC-ESI-MS/MS enabled large-scale identification of urinary proteomes, revealing distinct patterns related to arsenic exposure.
- These proteins represent potential diagnostic biomarkers for arsenic-induced bladder TCC.
Conclusions:
- Urinary proteomic analysis using HPLC-ESI-MS/MS is an effective method for discovering biomarkers of arsenic-associated bladder TCC.
- The identified proteins (ADAM, calpain9, ring finger protein 20) may serve as a panel for diagnosing bladder cancer linked to arsenic exposure.
- Further research can elucidate the precise mechanisms by which chronic arsenic exposure induces bladder cancer.
