Related Experiment Video
Updated: Jun 6, 2026
04:37
Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis
Published on: April 25, 2025
Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy
Eleni Giannakis1, Chrishan S Samuel, Tim D Hewitson
1Howard Florey Institute, The University of Melbourne, Victoria, Australia.
Proteomics. Clinical Applications
|December 8, 2010
Summary
Researchers identified novel protein biomarkers in blood and kidney tissue for early detection of renal fibrosis. This discovery could lead to non-invasive tests for kidney disease, improving patient outcomes and reducing the burden of kidney failure.
Area of Science:
- Biochemistry
- Proteomics
- Nephrology
Background:
- Kidney failure is a significant global health issue.
- Current diagnostic methods for kidney disease lack sensitivity and invasiveness.
- Early detection of renal fibrosis is crucial for effective intervention.
Purpose of the Study:
- To identify novel protein biomarkers for early detection of renal fibrosis.
- To develop a non-invasive clinical test for kidney disease.
- To understand the molecular pathology of obstructive nephropathy.
Main Methods:
- Utilized SELDI-TOF MS and 2-D gel electrophoresis for protein profiling.
- Screened plasma and kidney proteomes in a mouse model of unilateral ureteric obstruction.
- Employed immunohistochemistry to validate protein expression changes.
Main Results:
- Identified several differentially expressed proteins in plasma and kidney tissue, including serum amyloid A1, fibrinogen α, haptoglobin, and major urinary proteins.
- Confirmed up-regulation of fibrinogen through immunohistochemistry.
- Categorized identified proteins into acute-phase reactants, cell-signaling molecules, metabolic enzymes, and urinary proteins.
Conclusions:
- The identified proteins offer potential as biomarkers for early renal fibrosis detection.
- These findings provide insights into the pathology of obstructive nephropathy.
- Development of specific assays for these biomarkers could aid in monitoring kidney disease progression.
Related Concept Videos
01:29Acute Kidney Injury II: Pathophysiology
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
01:28Diabetic Nephropathy
Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...
01:10Nephrons
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma happens...
01:24Nephrotic Syndrome I : Introduction
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of fluid...
01:26Nephrotic Syndrome II : Assessment and Medical Management
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document any history...
01:25Chronic Kidney Disease I: Introduction
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...