Related Experiment Video

Updated: Jun 6, 2026

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

Proteomic and functional characterization of the outer membrane vesicles from the gastric pathogen Helicobacter

Erica Mullaney1, Paul A Brown, Sinead M Smith

  • 1Institute of Molecular Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.

Proteomics. Clinical Applications
|December 8, 2010
PubMed

Insights

Helicobacter pylori outer membrane vesicles (OMVs) carry proteins and toxins that mediate gastro-duodenal diseases. This study identified 162 proteins in H. pylori OMVs, revealing their role in host cell interactions.

Area of Science:

  • Microbiology
  • Pathogenesis
  • Proteomics

Background:

  • Helicobacter pylori is a gastric pathogen causing gastro-duodenal diseases.
  • Outer membrane vesicles (OMVs) shed by H. pylori are implicated in disease pathogenesis.
  • Understanding OMV composition is crucial for elucidating their role in host-pathogen interactions.

Purpose of the Study:

  • To determine the proteome of H. pylori OMVs.
  • To evaluate the mechanisms of OMV-mediated immuno-modulatory and cytotoxic activities.
  • To assess the effect of OMVs on gastric/colonic epithelial cells, duodenal explants, and neutrophils.

Main Methods:

  • Proteomic analysis of H. pylori OMVs using SDS-PAGE and LC-MS/MS.
  • Analysis of two H. pylori strains (J99 and NCTC 11637).

Main Results:

  • Identified 162 OMV-associated proteins in H. pylori strain J99 and 91 in NCTC 11637.
  • OMVs are rich in membrane proteins, porins, and adhesins.
  • OMVs carry cytotoxin-associated gene A (CagA) and vacuolating cytotoxin (VacA).

Conclusions:

  • H. pylori OMVs possess a proteome equipped with molecules for host cell interaction.
  • OMVs contribute to the pathogenesis of H. pylori-associated diseases.
  • OMVs function similarly to the intact pathogen in interacting with host cells.

More Related Videos

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance05:23

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance

Published on: March 7, 2025

High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability09:05

High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability

Published on: November 21, 2014

Related Concept Videos

Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...
Peptic Ulcer Disease II: Pathophysiology01:24

Peptic Ulcer Disease II: Pathophysiology

Peptic ulcer disease develops when protective mechanisms of the gastrointestinal mucosa are overwhelmed by harmful factors, leading to localized erosions in the stomach or proximal duodenum. The main causes are Helicobacter pylori infection and chronic use of nonsteroidal anti-inflammatory drugs (NSAIDs).Helicobacter pylori–Induced InjuryBacterial Adaptation and Colonization:H. pylori is a spiral, Gram-negative bacterium adapted to the acidic stomach. and transmitted through oral-oral or...
Peptic Ulcer01:27

Peptic Ulcer

Peptic ulcers are erosive lesions of the gastric or duodenal lining, most commonly caused by Helicobacter pylori infection. This Gram-negative, helical bacterium has adapted to survive the stomach’s acidic environment by producing urease, which converts urea into ammonia and carbon dioxide. The ammonia neutralizes gastric acid in the bacterium’s immediate environment, allowing colonization of the gastric mucosa. H. pylori attaches to mucus-secreting epithelial cells, penetrates the mucus...
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy01:16

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies01:28

Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies

Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...